Peer-Reviewed Journal Details
Mandatory Fields
PEMBROKE, JT; MCMAHON, M; SWEENEY, B
1995
March
Biotechnology Letters
PURIFICATION AND CHARACTERIZATION OF A CELLULOSE-BINDING ENDOXYLANASE FROM CELLULOMONAS-FLAVIGENA
Published
()
Optional Fields
DOMAINS XYLANASE
17
3
331
334
An extracellular endoxylanase capable of binding to avicel was isolated and purified from Cellulomonas flavigena. The purified xylanase, posessing no cellulose hydrolysing activity, had a molecular weight of 53K as determined electrophoretically. Steady state adsorption to avicel was reached within 5 minutes at 20 degrees C. The adsorption followed the Langmuir isotherm with a dissociation constant at 0 degrees C of 7.9E-8M and a saturation coefficient of 12 mg per gram with binding being stable for a wide range of ionic strengths down to 0.1M.
0141-5492
10.1007/BF01190648
Grant Details