Eight volunteers performed two bouts of 50 voluntary maximal eccentric contractions of the knee extensors of one leg 3 weeks apart. During maximal voluntary isometric contractions performed at intervals after each bout, electromyogram (EMG) mean power frequency declined after bout one (P < 0.01 Duncan's test), whereas integrated EMG did not change after either bout. These results suggest that unaccustomed eccentric contractions produce a temporary reduction in mean muscle activation frequency during subsequent maximal isometric contractions. (C) 1998 John Wiley & Sons, Inc.