Peer-Reviewed Journal Details
Mandatory Fields
Sioriki, E; Smith, TK; Demopoulos, CA; Zabetakis I.
Food Research International
Structure and cardioprotective activities of polar lipids of olive pomace, olive pomace-enriched fish feed and olive pomace fed gilthead sea bream (Sparus aurata)
In Press
Optional Fields
Gilthead sea bream (Sparus aurata); Olive pomace; Cardioprotective properties; Tandem mass spectrometry; Glycerophospholipids
Total lipids of olive pomace (OP), olive pomace diet (OP diet), fish oil diet (FO diet) and fish filets of farmed gilthead sea bream (fish fed with FO diet and OP diet respectively) were extracted and separated into polar (TPL) and neutral (TNL) lipids. All samples were assessed for their in vitro activity against washed rabbit platelets aggregation induced by platelet activating factor (PAF) and they were further analyzed by electrospray-mass spectrometry. The high levels of palmitic (16:0), oleic (18:1 cis ω − 9), linoleic (18:2 ω − 6) and docosapentaenoic acid (DPA 22:5 ω − 3) contained in both OP and FO diets are reflected to the gilthead sea breams fed with the individual diet respectively, while the gilthead sea bream fed with FO diet displays a decrease in DPA. All samples contained various glycerophospholipids species. Two PE species were identified in OP, OP diet and fish fed with OP diet and not in FO diet, while that might be an indication that these substances are likely to be the key polar phospholipids that have the ability to be in vitro PAF inhibitors, i.e. inhibit the formation of atherosclerotic plaques in blood arteries.
Grant Details
This work is supported in part by the aquaculture company NIREUS SA (grant Number 12407) and TKS's Wellcome Trust project grant 093228.