Peer-Reviewed Journal Details
Mandatory Fields
Holden, SA,Murphy, C,Moreno, JF,Butler, ST,Cromie, AR,Lonergan, P,Fair, S
2017
June
Reproduction Fertility And Development
In vitro characterisation of fresh and frozen sex-sorted bull spermatozoa
Published
()
Optional Fields
agglutination bovine gender bias oxidative stress sexed semen BOVINE SPERM BOAR SPERMATOZOA FERTILIZING ABILITY CONVENTIONAL SEMEN LIPID-PEROXIDATION HYDROGEN-PEROXIDE ACROSOME REACTION HOLSTEIN HEIFERS OXIDATIVE STRESS SORTING PROCESS
29
1415
1425
This study sought to compare the in vitro characteristics of fresh and frozen non-sorted (NS) and sex-sorted (SS) bull spermatozoa. Experiment 1: Holstein-Friesian ejaculates (n = 10 bulls) were split across four treatments and processed: (1) NS fresh at 3 x 10(6) spermatozoa, (2) X-SS frozen at 2 x 10(6) spermatozoa, (3) X-SS fresh at 2 x 10(6) spermatozoa and (4) X-SS fresh at 1 x 10(6) spermatozoa. NS frozen controls of 20 x 10(6) spermatozoa per straw were sourced from previously frozen ejaculates (n = 3 bulls). Experiment 2: Aberdeen Angus ejaculates (n = 4 bulls) were split across four treatments and processed as: (1) NS fresh 3 x 10(6) spermatozoa, (2) Y-SS fresh at 1 x 10(6) spermatozoa, (3) Y-SS fresh at 2 x 10(6) spermatozoa and (4) X-SS fresh at 2 x 10(6) spermatozoa. Controls were sourced as per Experiment 1. In vitro assessments for progressive linear motility, acrosomal status and oxidative stress were carried out on Days 1, 2 and 3 after sorting (Day 0 = day of sorting. In both experiments SS fresh treatments had higher levels of agglutination in comparison to the NS fresh (P < 0.001), NS frozen treatments had the greatest PLM (P < 0.05) and NS spermatozoa exhibited higher levels of superoxide anion production compared with SS spermatozoa (P < 0.05). Experiment 1 found both fresh and frozen SS treatments had higher levels of viable acrosome-intact spermatozoa compared with the NS frozen treatments (P < 0.01).
10.1071/RD16086
Grant Details