Peer-Reviewed Journal Details
Mandatory Fields
Mouras, R;Noor, MR;Pastorino, L;Bagnoli, E;Mani, A;Durack, E;Antipov, A;D'Autilia, F;Bianchini, P;Diaspro, A;Soulimane, T;Silien, C;Ruggiero, C;Tofail, SAM
2018
June
Acs Omega
Image-Based Tracking of Anticancer Drug-Loaded Nanoengineered Polyelectrolyte Capsules in Cellular Environments Using a Fast Benchtop Mid-Infrared (MIR) Microscope
Published
2 ()
Optional Fields
INFRARED MICROSPECTROSCOPY BIOMEDICAL APPLICATIONS SYNCHROTRON-RADIATION OPTICAL SPECTROSCOPY COLORIMETRIC ASSAY MICROCAPSULES ENCAPSULATION CAPABILITIES
3
6143
6150
Drug delivery monitoring and tracking in the human body are two of the biggest challenges in targeted therapy to be addressed by nanomedicine. The ability of imaging drugs and micro-/nanoengineered drug carriers and of visualizing their interactions at the cellular interface in a label-free manner is crucial in providing the ability of tracking their cellular pathways and will help understand their biological impact, allowing thus to improve the therapeutic efficacy. We present a fast, label-free technique to achieve high-resolution imaging at the mid-infrared (MIR) spectrum that provides chemical information. Using our custom-made benchtop infrared microscope using a high-repetition-rate pulsed laser (80 MHz, 40 ps), we were able to acquire images with subwavelength resolution (0.8 x lambda) at very high speeds. As a proof-of-concept, we embarked on the investigation of nanoengineered polyelectrolyte capsules (NPCs) containing the anticancer drug, docetaxel. These NPCs were synthesized using a layer-by-layer approach built upon a calcium carbonate (CaCO3) core, which was then removed away with ethylenediaminetetraacetic acid. The obtained MIR images show that NPCs are attached to the cell membrane, which is a good step toward an efficient drug delivery. This has been confirmed by both three-dimensional confocal fluorescence and stimulated emission depletion microscopy. Coupled with additional instrumentation and data processing advancements, this setup is capable of video-rate imaging speeds and will be significantly complementing current super-resolution microscopy techniques while providing an unperturbed view into living cells.
WASHINGTON
2470-1343
10.1021/acsomega.7b01859
Grant Details