Peer-Reviewed Journal Details
Mandatory Fields
Wang J.;Xiang X.;Milcovich G.;Chen J.;Chen C.;Feng J.;Hudson S.;Weng X.;Ruan Y.
2019
February
Journal Of Molecular Recognition
Nitrogen and sulfur co-doped carbon nanodots toward bovine hemoglobin: A fluorescence quenching mechanism investigation
Published
12 ()
Optional Fields
bovine hemoglobin fluorescence nitrogen and sulfur co-doped carbon dots quenching mechanism
32
2
© 2018 John Wiley & Sons, Ltd. A deep understanding of the molecular interactions of carbon nanodots with biomacromolecules is essential for wider applications of carbon nanodots both in vitro and in vivo. Herein, nitrogen and sulfur co-doped carbon dots (N,S-CDs) with a quantum yield of 16% were synthesized by a 1-step hydrothermal method. The N,S-CDs exhibited a good dispersion, with a graphite-like structure, along with the fluorescence lifetime of approximately 7.50 ns. Findings showed that the fluorescence of the N,S-CDs was effectively quenched by bovine hemoglobin as a result of the static fluorescence quenching. The mentioned quenching mechanism was investigated by the Stern-Volmer equation, temperature-dependent quenching, and fluorescence lifetime measurements. The binding constants, number of binding sites, and the binding average distance between the energy donor N,S-CDs and acceptor bovine hemoglobin were calculated as well. These findings will provide for valuable insights on the future bioapplications of N,S-CDs.
0952-3499
10.1002/jmr.2761
Grant Details