Peer-Reviewed Journal Details
Mandatory Fields
Avila Rodriguez, M.,Garcia-Segura, L. M.,Cabezas, R.,Torrente, D.,Capani, F.,Gonzalez, J.,Barreto, G. E.
2014
October
J Steroid Biochem Mol Biolj Steroid Biochem Mol Biol
Tibolone protects T98G cells from glucose deprivation
Published
()
Optional Fields
Astrocytes/*drug effects/metabolism/physiology Calcium/metabolism Cell Death/drug effects Cell Line Estrogen Receptor Modulators/*pharmacology Glucose/*metabolism Humans Membrane Potential, Mitochondrial/drug effects Neuroprotective Agents/*pharmacology Norpregnenes/*pharmacology Reactive Oxygen Species/metabolism
144 Pt B
294
303
The steroidal drug Tibolone is used for the treatment of climacteric symptoms and osteoporosis in post-menopausal women. Although Tibolone has been shown to exert neuroprotective actions after middle cerebral artery occlusion, its specific actions on glial cells have received very little attention. In the present study we have assessed whether Tibolone exerts protective actions in a human astrocyte cell model, the T98G cells, subjected to glucose deprivation. Our findings indicate that Tibolone decreases the effects of glucose deprivation on cell death, nuclear fragmentation, superoxide ion production, mitochondrial membrane potential, cytoplasmic calcium concentration and morphological parameters. These findings suggest that glial cells may participate in the neuroprotective actions of Tibolone in the brain.The steroidal drug Tibolone is used for the treatment of climacteric symptoms and osteoporosis in post-menopausal women. Although Tibolone has been shown to exert neuroprotective actions after middle cerebral artery occlusion, its specific actions on glial cells have received very little attention. In the present study we have assessed whether Tibolone exerts protective actions in a human astrocyte cell model, the T98G cells, subjected to glucose deprivation. Our findings indicate that Tibolone decreases the effects of glucose deprivation on cell death, nuclear fragmentation, superoxide ion production, mitochondrial membrane potential, cytoplasmic calcium concentration and morphological parameters. These findings suggest that glial cells may participate in the neuroprotective actions of Tibolone in the brain.
1879-1220 (Electronic) 09
2014/08/03
http://www.ncbi.nlm.nih.gov/pubmed/25086299http://www.ncbi.nlm.nih.gov/pubmed/25086299
10.1016/j.jsbmb.2014.07.009
Grant Details