© 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/) Fly ash from a poultry litter gasification process and the potential of application of the fly ash as a fertiliser in line with the poultry litter protocol is investigated. The fines collected in the cyclone are mainly formed by ash which comprises between 70-83 wt.% of the fines on a dry basis, and to a lesser extent of carbon (elutriated char). The effect of the gasification operating conditions on the concentration of ash forming elements (inorganic compounds) in the fly ash, are discussed. In addition, the enrichment factor which defines the volatility, has been used and fly ash elements were categorised as Class I (non-volatile), Class II (semi-volatile with the possible occurrence of condensation) and Class III (highly volatile elements). Inorganic elements in fly ashes from poultry litter gasification experiments are categorised as Class I: Ca, Co, Cu, Fe, Hg, K, Mg, Mn, Na, Ni, P, Class II: Cd, Cr, Mo and Class III: Pb and Se. It has been found that the fly ash from the poultry litter gasification exceeds the upper acceptable limit set by Poultry Litter Protocol to be used as a fertiliser in agriculture systems.