Peer-Reviewed Journal Details
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Davies R.W.;Bass J.J.;Carson B.P.;Norton C.;Kozior M.;Wilkinson D.J.;Brook M.S.;Atherton P.J.;Smith K.;Jakeman P.M.
The effect of whey protein supplementation on myofibrillar protein synthesis and performance recovery in resistance-trained men
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Optional Fields
Deuterium oxide Dietary protein Exercise performance Humans Skeletal muscle Whey protein
2020 by the authors. Licensee MDPI, Basel, Switzerland. Background: The aim of this study was to investigate the effect of whey protein supplementation on myofibrillar protein synthesis (myoPS) and muscle recovery over a 7-d period of intensified resistance training (RT). Methods: In a double-blind randomised parallel group design, 16 resistance-trained men aged 18 to 35 years completed a 7-d RT protocol, consisting of three lower-body RT sessions on non-consecutive days. Participants consumed a controlled diet (146 kJkg1d1, 1.7 gkg1d1 protein) with either a whey protein supplement or an isonitrogenous control (0.33 gkg1d1 protein). To measure myoPS, 400 ml of deuterium oxide (D2 O) (70 atom %) was ingested the day prior to starting the study and m. vastus lateralis biopsies were taken before and after RT-intervention. Myofibrillar fractional synthetic rate (myoFSR) was calculated via deuterium labelling of myofibrillar-bound alanine, measured by gas chromatography-pyrolysis-isotope ratio mass spectrometry (GC-Pyr-IRMS). Muscle recovery parameters (i.e., countermovement jump height, isometric-squat force, muscle soreness and serum creatine kinase) were assessed daily. Results: MyoFSR PRE was 1.6 (0.2) %d1 (mean (SD)). Whey protein supplementation had no effect on myoFSR (p = 0.771) or any recovery parameter (p = 0.3900.989). Conclusions: Over an intense 7-d RT protocol, 0.33 gkg1d1 of supplemental whey protein does not enhance day-to-day measures of myoPS or postexercise recovery in resistance-trained men.
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