Iron, zinc, copper, manganese, molybdenum, cobalt, and possibly, chromium are essential trace metals for humans. Zinc, in particular, is vital for many physiological processes that regulate endocrine and immune functions and control healthy brain development and activity. However, currently, it is estimated that 20 percent of the human population is at risk of zinc deficiency. Therefore, zinc supplementation became a significant focus in trace metal and health research, acting as a nutritional supplement to compensate for low zinc bioavailability and nutraceutical with pharmacological activity. This chapter will provide background to the nutritional zinc requirements of humans, introduce zinc supplements such as inorganic and organic supplements and discuss their differences and usefulness in various applications. In addition, the chapter will summarize scientifically proven beneficial effects of zinc supplementation and highlight new potential areas for zinc supplementation as a prevention and treatment strategy.